Response surface methodology study of the combined effects of temperature, pH, and aw on the growth rate of Trichoderma asperellum.
Identifieur interne : 001D97 ( Main/Exploration ); précédent : 001D96; suivant : 001D98Response surface methodology study of the combined effects of temperature, pH, and aw on the growth rate of Trichoderma asperellum.
Auteurs : B A D. Begoude [Cameroun] ; R. Lahlali ; D. Friel ; P R Tondje ; M H JijakliSource :
- Journal of applied microbiology [ 1364-5072 ] ; 2007.
Descripteurs français
- KwdFr :
- Concentration en ions d'hydrogène (MeSH), Eau (MeSH), Glycérol (MeSH), Lutte biologique contre les nuisibles (méthodes), Microbiologie du sol (MeSH), Milieux de culture (composition chimique), Modèles biologiques (MeSH), Température (MeSH), Trichoderma (classification), Trichoderma (croissance et développement).
- MESH :
- composition chimique : Milieux de culture, Trichoderma.
- croissance et développement : Trichoderma.
- méthodes : Lutte biologique contre les nuisibles.
- Concentration en ions d'hydrogène, Eau, Glycérol, Microbiologie du sol, Modèles biologiques, Température.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Culture Media.
- chemical : Glycerol, Water.
- classification : Trichoderma.
- growth & development : Trichoderma.
- methods : Pest Control, Biological.
- Hydrogen-Ion Concentration, Models, Biological, Soil Microbiology, Temperature.
Abstract
AIMS
To evaluate the influence of environmental parameters (water activity aw, temperature, and pH) on the radial growth rate of Trichoderma asperellum (strains PR10, PR11, PR12, and 659-7), an antagonist of Phytophthora megakarya, the causal agent of cocoa black pod disease.
METHODS AND RESULTS
The radial growth of four strains of T. asperellum was monitored for 30 days on modified PDA medium. Six levels of aw (0.995, 0.980, 0.960, 0.930, 0.910, and 0.880) were combined with three values of pH (4.5, 6.5, and 8.5) and three incubation temperatures (20, 25, and 30 degrees C). Whatever the strain, mycelial growth rate was optimal at aw between 0.995 and 0.980, independently of the temperature and pH. Each strain appeared to be very sensitive to aw reduction. In addition, all four strains were able to grow at all temperatures and pH values (4.5-8.5) tested, highest growth rate being observed at 30 degrees C and at pH 4.5-6.5. The use of response surface methodology to model the combined effects of aw, temperature, and pH on the radial growth rate of the T. asperellum strains confirmed the observed results. In our model, growth of the T. asperellum strains showed a greater dependence on aw than on temperature or pH under in vitro conditions.
CONCLUSION
aw is a crucial environmental factor. Low aw can prevent growth of T. asperellum strains under some conditions. The observed and predicted radial growth rate of strain PR11 showed its greater capacity to support low aw (0.93) as compared with other tested strains at 20 degrees C. This is in agreement with its better protective level when applied in medium-scale trials on cocoa plantations.
SIGNIFICANCE AND IMPACT OF THE STUDY
This study should contribute towards improving the biocontrol efficacy of T. asperellum strains used against P. megakarya. Integrated into a broader study of the impact of environmental factors on the biocontrol agent-pathogen system, this work should help to build a more rational control strategy, possibly involving the use of a compatible adjuvant protecting T. asperellum against desiccation.
DOI: 10.1111/j.1365-2672.2007.03305.x
PubMed: 17897186
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Response surface methodology study of the combined effects of temperature, pH, and aw on the growth rate of Trichoderma asperellum.</title><author><name sortKey="Begoude, B A D" sort="Begoude, B A D" uniqKey="Begoude B" first="B A D" last="Begoude">B A D. Begoude</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire Régional de Lutte Biologique et de Microbiologie Appliquée, Institut de le Recherche Agricole pour le Développement (IRAD), Nkolbisson, BP 2067, Yaoundé, Cameroon.</nlm:affiliation><country xml:lang="fr">Cameroun</country><wicri:regionArea>Laboratoire Régional de Lutte Biologique et de Microbiologie Appliquée, Institut de le Recherche Agricole pour le Développement (IRAD), Nkolbisson, BP 2067, Yaoundé</wicri:regionArea><wicri:noRegion>Yaoundé</wicri:noRegion></affiliation></author><author><name sortKey="Lahlali, R" sort="Lahlali, R" uniqKey="Lahlali R" first="R" last="Lahlali">R. Lahlali</name></author><author><name sortKey="Friel, D" sort="Friel, D" uniqKey="Friel D" first="D" last="Friel">D. Friel</name></author><author><name sortKey="Tondje, P R" sort="Tondje, P R" uniqKey="Tondje P" first="P R" last="Tondje">P R Tondje</name></author><author><name sortKey="Jijakli, M H" sort="Jijakli, M H" uniqKey="Jijakli M" first="M H" last="Jijakli">M H Jijakli</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2007">2007</date><idno type="RBID">pubmed:17897186</idno><idno type="pmid">17897186</idno><idno type="doi">10.1111/j.1365-2672.2007.03305.x</idno><idno type="wicri:Area/Main/Corpus">001F14</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001F14</idno><idno type="wicri:Area/Main/Curation">001F14</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001F14</idno><idno type="wicri:Area/Main/Exploration">001F14</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Response surface methodology study of the combined effects of temperature, pH, and aw on the growth rate of Trichoderma asperellum.</title><author><name sortKey="Begoude, B A D" sort="Begoude, B A D" uniqKey="Begoude B" first="B A D" last="Begoude">B A D. Begoude</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire Régional de Lutte Biologique et de Microbiologie Appliquée, Institut de le Recherche Agricole pour le Développement (IRAD), Nkolbisson, BP 2067, Yaoundé, Cameroon.</nlm:affiliation><country xml:lang="fr">Cameroun</country><wicri:regionArea>Laboratoire Régional de Lutte Biologique et de Microbiologie Appliquée, Institut de le Recherche Agricole pour le Développement (IRAD), Nkolbisson, BP 2067, Yaoundé</wicri:regionArea><wicri:noRegion>Yaoundé</wicri:noRegion></affiliation></author><author><name sortKey="Lahlali, R" sort="Lahlali, R" uniqKey="Lahlali R" first="R" last="Lahlali">R. Lahlali</name></author><author><name sortKey="Friel, D" sort="Friel, D" uniqKey="Friel D" first="D" last="Friel">D. Friel</name></author><author><name sortKey="Tondje, P R" sort="Tondje, P R" uniqKey="Tondje P" first="P R" last="Tondje">P R Tondje</name></author><author><name sortKey="Jijakli, M H" sort="Jijakli, M H" uniqKey="Jijakli M" first="M H" last="Jijakli">M H Jijakli</name></author></analytic><series><title level="j">Journal of applied microbiology</title><idno type="ISSN">1364-5072</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Culture Media (chemistry)</term><term>Glycerol (MeSH)</term><term>Hydrogen-Ion Concentration (MeSH)</term><term>Models, Biological (MeSH)</term><term>Pest Control, Biological (methods)</term><term>Soil Microbiology (MeSH)</term><term>Temperature (MeSH)</term><term>Trichoderma (classification)</term><term>Trichoderma (growth & development)</term><term>Water (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Concentration en ions d'hydrogène (MeSH)</term><term>Eau (MeSH)</term><term>Glycérol (MeSH)</term><term>Lutte biologique contre les nuisibles (méthodes)</term><term>Microbiologie du sol (MeSH)</term><term>Milieux de culture (composition chimique)</term><term>Modèles biologiques (MeSH)</term><term>Température (MeSH)</term><term>Trichoderma (classification)</term><term>Trichoderma (croissance et développement)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Culture Media</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Glycerol</term><term>Water</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Trichoderma</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Milieux de culture</term><term>Trichoderma</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Trichoderma</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Trichoderma</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Pest Control, Biological</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Lutte biologique contre les nuisibles</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Hydrogen-Ion Concentration</term><term>Models, Biological</term><term>Soil Microbiology</term><term>Temperature</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Concentration en ions d'hydrogène</term><term>Eau</term><term>Glycérol</term><term>Microbiologie du sol</term><term>Modèles biologiques</term><term>Température</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>AIMS</b></p><p>To evaluate the influence of environmental parameters (water activity aw, temperature, and pH) on the radial growth rate of Trichoderma asperellum (strains PR10, PR11, PR12, and 659-7), an antagonist of Phytophthora megakarya, the causal agent of cocoa black pod disease.</p></div><div type="abstract" xml:lang="en"><p><b>METHODS AND RESULTS</b></p><p>The radial growth of four strains of T. asperellum was monitored for 30 days on modified PDA medium. Six levels of aw (0.995, 0.980, 0.960, 0.930, 0.910, and 0.880) were combined with three values of pH (4.5, 6.5, and 8.5) and three incubation temperatures (20, 25, and 30 degrees C). Whatever the strain, mycelial growth rate was optimal at aw between 0.995 and 0.980, independently of the temperature and pH. Each strain appeared to be very sensitive to aw reduction. In addition, all four strains were able to grow at all temperatures and pH values (4.5-8.5) tested, highest growth rate being observed at 30 degrees C and at pH 4.5-6.5. The use of response surface methodology to model the combined effects of aw, temperature, and pH on the radial growth rate of the T. asperellum strains confirmed the observed results. In our model, growth of the T. asperellum strains showed a greater dependence on aw than on temperature or pH under in vitro conditions.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSION</b></p><p>aw is a crucial environmental factor. Low aw can prevent growth of T. asperellum strains under some conditions. The observed and predicted radial growth rate of strain PR11 showed its greater capacity to support low aw (0.93) as compared with other tested strains at 20 degrees C. This is in agreement with its better protective level when applied in medium-scale trials on cocoa plantations.</p></div><div type="abstract" xml:lang="en"><p><b>SIGNIFICANCE AND IMPACT OF THE STUDY</b></p><p>This study should contribute towards improving the biocontrol efficacy of T. asperellum strains used against P. megakarya. Integrated into a broader study of the impact of environmental factors on the biocontrol agent-pathogen system, this work should help to build a more rational control strategy, possibly involving the use of a compatible adjuvant protecting T. asperellum against desiccation.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17897186</PMID><DateCompleted><Year>2008</Year><Month>04</Month><Day>14</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1364-5072</ISSN><JournalIssue CitedMedium="Print"><Volume>103</Volume><Issue>4</Issue><PubDate><Year>2007</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Journal of applied microbiology</Title><ISOAbbreviation>J Appl Microbiol</ISOAbbreviation></Journal><ArticleTitle>Response surface methodology study of the combined effects of temperature, pH, and aw on the growth rate of Trichoderma asperellum.</ArticleTitle><Pagination><MedlinePgn>845-54</MedlinePgn></Pagination><Abstract><AbstractText Label="AIMS" NlmCategory="OBJECTIVE">To evaluate the influence of environmental parameters (water activity aw, temperature, and pH) on the radial growth rate of Trichoderma asperellum (strains PR10, PR11, PR12, and 659-7), an antagonist of Phytophthora megakarya, the causal agent of cocoa black pod disease.</AbstractText><AbstractText Label="METHODS AND RESULTS" NlmCategory="RESULTS">The radial growth of four strains of T. asperellum was monitored for 30 days on modified PDA medium. Six levels of aw (0.995, 0.980, 0.960, 0.930, 0.910, and 0.880) were combined with three values of pH (4.5, 6.5, and 8.5) and three incubation temperatures (20, 25, and 30 degrees C). Whatever the strain, mycelial growth rate was optimal at aw between 0.995 and 0.980, independently of the temperature and pH. Each strain appeared to be very sensitive to aw reduction. In addition, all four strains were able to grow at all temperatures and pH values (4.5-8.5) tested, highest growth rate being observed at 30 degrees C and at pH 4.5-6.5. The use of response surface methodology to model the combined effects of aw, temperature, and pH on the radial growth rate of the T. asperellum strains confirmed the observed results. In our model, growth of the T. asperellum strains showed a greater dependence on aw than on temperature or pH under in vitro conditions.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">aw is a crucial environmental factor. Low aw can prevent growth of T. asperellum strains under some conditions. The observed and predicted radial growth rate of strain PR11 showed its greater capacity to support low aw (0.93) as compared with other tested strains at 20 degrees C. This is in agreement with its better protective level when applied in medium-scale trials on cocoa plantations.</AbstractText><AbstractText Label="SIGNIFICANCE AND IMPACT OF THE STUDY" NlmCategory="CONCLUSIONS">This study should contribute towards improving the biocontrol efficacy of T. asperellum strains used against P. megakarya. Integrated into a broader study of the impact of environmental factors on the biocontrol agent-pathogen system, this work should help to build a more rational control strategy, possibly involving the use of a compatible adjuvant protecting T. asperellum against desiccation.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Begoude</LastName><ForeName>B A D</ForeName><Initials>BA</Initials><AffiliationInfo><Affiliation>Laboratoire Régional de Lutte Biologique et de Microbiologie Appliquée, Institut de le Recherche Agricole pour le Développement (IRAD), Nkolbisson, BP 2067, Yaoundé, Cameroon.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lahlali</LastName><ForeName>R</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Friel</LastName><ForeName>D</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Tondje</LastName><ForeName>P R</ForeName><Initials>PR</Initials></Author><Author ValidYN="Y"><LastName>Jijakli</LastName><ForeName>M H</ForeName><Initials>MH</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Appl Microbiol</MedlineTA><NlmUniqueID>9706280</NlmUniqueID><ISSNLinking>1364-5072</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003470">Culture Media</NameOfSubstance></Chemical><Chemical><RegistryNumber>059QF0KO0R</RegistryNumber><NameOfSubstance UI="D014867">Water</NameOfSubstance></Chemical><Chemical><RegistryNumber>PDC6A3C0OX</RegistryNumber><NameOfSubstance UI="D005990">Glycerol</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D003470" MajorTopicYN="N">Culture Media</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005990" MajorTopicYN="N">Glycerol</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006863" MajorTopicYN="N">Hydrogen-Ion Concentration</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008954" MajorTopicYN="N">Models, Biological</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010572" MajorTopicYN="N">Pest Control, Biological</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012988" MajorTopicYN="N">Soil Microbiology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013696" MajorTopicYN="N">Temperature</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014242" MajorTopicYN="N">Trichoderma</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014867" MajorTopicYN="Y">Water</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2007</Year><Month>9</Month><Day>28</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2008</Year><Month>4</Month><Day>15</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2007</Year><Month>9</Month><Day>28</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">17897186</ArticleId><ArticleId IdType="pii">JAM3305</ArticleId><ArticleId IdType="doi">10.1111/j.1365-2672.2007.03305.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Cameroun</li></country></list><tree><noCountry><name sortKey="Friel, D" sort="Friel, D" uniqKey="Friel D" first="D" last="Friel">D. Friel</name><name sortKey="Jijakli, M H" sort="Jijakli, M H" uniqKey="Jijakli M" first="M H" last="Jijakli">M H Jijakli</name><name sortKey="Lahlali, R" sort="Lahlali, R" uniqKey="Lahlali R" first="R" last="Lahlali">R. Lahlali</name><name sortKey="Tondje, P R" sort="Tondje, P R" uniqKey="Tondje P" first="P R" last="Tondje">P R Tondje</name></noCountry><country name="Cameroun"><noRegion><name sortKey="Begoude, B A D" sort="Begoude, B A D" uniqKey="Begoude B" first="B A D" last="Begoude">B A D. Begoude</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhytophthoraV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001D97 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001D97 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhytophthoraV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:17897186
|texte= Response surface methodology study of the combined effects of temperature, pH, and aw on the growth rate of Trichoderma asperellum.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:17897186" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhytophthoraV1
| This area was generated with Dilib version V0.6.38. |  |